Figure 1. Rescue of cardiac alpha-actin-deficient mice by enteric smooth muscle gamma-actin. Restricted beta-galactosidase expression of a hygromycin-lacZ gene targeted to the beta-actin locus and embryonic lethality of beta-actin mutant mice. Smooth muscle (SM) -actin is a useful gene for studying transcriptional regulation of the SMC differentiation program. The significance of these transitions in actin gene expression during myogenesis remains to be determined. ACTA2 (as with all the actins) is extremely highly conserved and found in nearly all mammals. We confirmed in vitro, and in an in vivo bleomycin model of lung fibrosis, that CO suppresses -smooth muscle actin expression and enhances small proline-rich protein-1a expression . ACTA2 (Actin Alpha 2, Smooth Muscle) is a Protein Coding gene. Reacts with tumors arising from smooth muscles and myoepithelial cells. Their emergence can be induced by cytokines such as transforming growth factor beta; but the regulatory mechanism for induction of alpha-smooth muscle actin gene expression Szucsik and Lessard (1995) characterized the mouse smooth muscle (enteric) gamma-actin gene. Motility in all cells is also partly based on similar interactions of actin filaments with non-muscle myosins. Gene expression was analyzed using low-density arrays and confirmed by immunostaining. Given previous developments in molecular biology, gene therapies inhibiting smooth muscle cell proliferation have become important for RS treatment . Excessive accumulation of alpha smooth muscle actin-positive (ACTA2+) activated myofibroblasts is observed in Idiopathic pulmonary fibrosis (2). The MLCK probe was a 50-bp segment corresponding to the 5 end of human smooth muscle MLCK cDNA. Anti-alpha-Actin Antibody, Smooth Muscle is an antibody against alpha-Actin for use in Western Blotting, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation. Disease related genes Human disease related genes Plasma proteins: Predicted location i. Intracellular: Number of . Excessive accumulation of alpha smooth muscle actin-positive (ACTA2+) activated myofibroblasts is observed in Idiopathic pulmonary fibrosis (2). It is a member of the Actin family. Alpha-smooth muscle actin is abundant in vascular and visceral smooth muscle cells. Neointima formation in TV is the accumulation of -smooth muscle actin (-SMA)-positive smooth muscle cells (SMCs) between the endothelial lining and the internal . Smooth muscle alpha actin is of further interest because it is one of a few genes whose expression is relatively restricted to vascular smooth muscle cells. To identify potential regulators of smooth muscle cell (SMC) differentiation, we studied the molecular mechanisms that control the tissue-specific transcriptional expression of SM -actin, the most abundant protein in fully differentiated SMCs. My Genes Showing 25 of 820 Results for Alpha-Smooth Muscle Actin antibodies Search Time: 0 ms Showing 25 50 100 200 500 See All of 820 Results for Alpha-Smooth Muscle Actin antibodies Search Time: 0 ms The dynamic properties of the actin cytoskeleton in smooth muscle cells play an important role in a number of cardiovascular disease states. These contractions empty urine from the bladder and move food through the intestines as part of the digestive process. Multiple alternatively spliced variants, encoding the same protein, have been identified. The three muscle cell types, skeletal, cardiac, and smooth, are all derived from distinct populations of myogenic precursor cells during embryogenesis. GTX100034 Reference 75 7 Datasheet File See all alpha Smooth Muscle Actin products APPLICATION Application Note *Optimal dilutions/concentrations should be determined by the researcher. View List. Not tested in other applications. Alpha-Smooth Muscle Actin was detected in paraffin-embedded section of rat cardiac muscle tissues. Alpha smooth muscle actin (-SM-actin) immunocytochemical staining revealed an abundance of tan-yellow myonemes in the cytoplasm, which were aligned parallel to the vertical axis of the cells. SDS CoA Smooth muscle alpha-actin gene requires two E-boxes for proper expression in vivo and is a target of class I basic helix-loop-helix proteins. The muscular tissue is contractile, i.e., it can contract and relax. A 240-bp fragment from human airway -smooth muscle actin (position 1057-1297) was amplified by reverse transcription-polymerase chain reaction. Smooth Muscle Actin Antibody (CGA7) is recommended for detection of and Actin isotypes of smooth muscle cells of mouse, rat, human and avian origin by WB, IP, IF, IHC (P) and ELISA; non cross-reactive with skeletal or cardiac cells No. The secondary antibody ImmPRESS HRP anti-mouse IgG was incubated for 20 min at RT. Remove. Published: 19 March 1998; Human smooth muscle -actin gene is a transcriptional . Liver injury, associated with cirrhosis, induces transformation of resident hepatic stellate cells into liver specific myofibroblasts, also known as activated cells. -Smooth muscle actin is first and transiently expressed, followed by -skeletal and finally -cardiac actin. View more details Although actin isoforms are . They combine untrastructural and functional features of SMCs and fibroblasts. custom hamburgers near me; best 15 inch off-road tires; integrated project delivery method pros and cons. Smooth Muscle Actin ( Figures 25-3, A, and 25-4, A) The smooth muscle actin (SMA) marker has a relatively long track record of use as a myoepithelial marker in breast pathology diagnosis. Smooth muscle alpha actin is one of a few genes whose expression is predominantly expressed in vascular smooth muscle cells that is used to study myofibroblasts and fibrosis (1). This study, as an initial step toward our goal, was aimed to isolate genetic determinants for three representative muscle actins (skeletal, cardiac, and smooth muscle types) from mud loach and to examine the isoform-dependent expression patterns in adult tissues and developing embryos and larvae. Behringer RR. Smooth Muscle Differentiation Marker Gene Expression Is Regulated by RhoA-mediated Actin Polymerization* Smooth muscle cell (SMC) differentiation is regulated by a complex array of local environmental cues, but the intracellular signaling pathways and the transcription mechanisms that regulate this process are largely unknown. In the relaxed state, each cell is 30-200 micrometers in length, some thousands of times shorter than a skeletal muscle cell. Further more, expression of smooth muscle alpha actin is regulated by hormones, cell proliferation and altered by pathological conditions including oncogenic transformation and . ACTA2 is one of 6 different actin isoforms and is involved in the contractile apparatus of smooth muscle. Smooth muscle alpha actin is one of a few genes whose expression is predominantly expressed in vascular smooth muscle cells that is used to study myofibroblasts and fibrosis (1). Thermal stability of the hybrids and sensitivity to S1 digestion also indicated substantial divergence between the 3' untranslated end of rat and dog skeletal muscle actins. The show/hide columns function can be used to customize the columns in the search result list. Add to Procurement List Product is on your procurement list. The muscular tissues are of three types: skeletal muscles, smooth muscles, and cardiac muscles. Ca 2+ CLEARANCE AND REGULATION OF [Ca 2+] i. Ca 2+ homeostasis is central to the regulation of smooth muscle function. A mouse monoclonal antibody, raised against Actin, aortic smooth muscle, supplied by MONOSAN. The basic mechanism in muscle, including heart muscle, involves the interaction of the protein filaments myosin and actin. Human smooth muscle -actin gene is a transcriptional target of the p53 tumor suppressor protein Download PDF. 12 Here we demonstrate that Notch-mediated upregulation of SMA is directly dependent on the activation and binding of CSL to the SMA promoter. ACTA2 is the isoform of contractile protein alpha-actin present in vascular smooth muscle cells (SMCs) throughout the arterial tree.4 Pathologic conditions in individuals with ACTA2 mutations show increased deposition of SMCs in the intimal arterial layer, leading to a decreased intraluminal diameter.4 The investigation shows that the coding regions of actin genes are highly conserved, whereas the 3' non-coding regions diverged considerably during evolution. This antibody could be used together with Muscle-Specific Actin to distinguish Leiomyosarcoma from Rhabdomyosarcoma. -smooth muscle actin, encoded by ACTA2 gene, is an isoform of the vascular smooth muscle actins, typically expressed in the vascular smooth muscle cells contributing to vascular motility and contraction. Listed are ELISA Kits for the detection of alpha smooth muscle Actin, an alias name of actin alpha 2, smooth muscle. SM -actin is the first SMC differentiation marker to appear during development, 1 and although it is transiently expressed in cardiac and skeletal muscle during development and in myofibroblasts during wound healing, 1 its . Smooth muscles line the internal organs; they contract and relax without being consciously controlled. This gene encodes actin gamma 2\; a smooth muscle actin found in enteric tissues. Smooth muscle -2 actin is found in smooth muscle cells. continental double fighter iii 700c; what are the 3 types of embroidery needles? The gene encodes a mature actin protein of 374 amino acids. 35) In a skeletal muscle cell, extensions of the myosin molecules called _____ can bind to grooves in the actin proteins. SMA is the most abundant protein in SMC and appears to play an important role in mechanotransduction and generation of traction forces in SMC as well as myofibroblasts. The most highly differentially expressed categories of genes included those related to muscular system development and the small proline-rich family of proteins. IHC analysis of Alpha-Smooth Muscle Actin using anti- Alpha-Smooth Muscle Actin antibody (MA1106). A smooth muscle cell is a spindle-shaped myocyte with a wide middle and tapering ends, and a single nucleus. This protein is reported to have a cytoplasmic cellular localization. PROPERTIES TARGET DATA IMAGES REFERENCE REVIEW Add to Cart Add to Cart Antibody Validation Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity. A construct containing the region from 1 to 125 of the promoter (p125CAT) had high transcriptional activity in SMCs (57-fold > promoterless) and . Gene Acta2 View Product On Supplier's Website. 15 Methods Proc Natl Acad Sci USA. Vascular smooth muscle cells (VSMCs) are the main stromal cells in the medial layer of the vascular wall. Transgenic Res . . In allografts, neointimal SMCs expressed increased levels of Tgf 1 and Pdgfb . These antibodies target Alpha-Smooth Muscle Actin in Human, Mouse, Rat, Chicken and Non-human primate samples. Background Congenital mydriasis and retinal arteriolar tortuosity are associated with the life-threatening multisystemic smooth muscle dysfunction syndrome (MSMDS) due to mutations in the gene, ACTA2, which encodes alpha-smooth muscle actin (-SMA). Publications (8) 1 Infection of Human Retinal Pigment Epithelium with Chlamydia trachomatis. Menu Sign in or Register Search; . Different interacting signaling modules involving Ca 2+ /calmodulin-dependent myosin light chain kinase, Ca 2+-independent regulatory light chain phosphorylation, myosin phosphatase inhibition, and actin filament-based proteins are proposed as specific cellular mechanisms involved in the regulation of smooth muscle contraction.However, the relative importance of specific modules is not well . In contrast, in . Answer and Explanation: 1 -smooth muscle actin, encoded by ACTA2 gene, is an isoform of the vascular smooth muscle actins, typically expressed in the vascular smooth muscle cells contributing to vascular motility and contraction. Anti-alpha-Actin Antibody, Smooth Muscle MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents. Beside -SMA, -actin has a special importance also, as it is a widely used internal control in many molecular biological measurements. The -2 actin protein is necessary for contraction of the smooth muscles in the bladder and intestines. 36) When the muscle cell is relaxed, the myosin heads are blocked from binding to actin because a protein called _____ blocks the binding sites on the actin. Semicarbazide-sensitive amine oxidase (SSAO) and lysyl oxidase (LOX) are vascular enzymes associated with the development of atherosclerosis. We use cookies to enhance the usability of our website. This actin is an alpha actin that is found in skeletal muscle. 1997; 94 (9):4406-4411. The two most commonly used antibodies are anti-SMA and muscle-specific actin (clone HHF-35). Smooth muscle is composed of sheets or strands of smooth muscle cells. Actin alpha 2, smooth muscle: Protein class i. The human protein, encoded by the gene ACTA2, is 377 amino acid residues long and has a mass of 42,009 daltons. The state of actin does not only mediate mechanical stability and contractile function but can also regulate gene expression via myocardin related transcription factors (MRTFs). Product Note Recognizes human alpha-smooth muscle isoform of actin. Diseases associated with ACTA2 include Multisystemic Smooth Muscle Dysfunction Syndrome and Aortic Aneurysm, Familial Thoracic 6 . Defects in this gene cause aortic aneurysm familial thoracic type 6. GO analysis in bladder tissue showed prominent increases in biological pathways involved in cell proliferation, metabolism, actin cytoskeleton and myosin, as well as decreases in cell motility, and regulation of muscle contraction. Summary of ACTA2 (ACTSA) expression in human tissue. Smooth muscle actin belongs to the actin gene family consisting six different isoforms also including -cardiac- and -skeletal-actin, -actin, -cyto- and -smooth-actin. [PMC free article] . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. 690001) was diluted in PBS (antibody concentration 400 ng/ml) and incubated at 4C over-night. Selective expression in smooth muscle cells and myoepithelial cells. It is well established that [Ca 2+] i plays an essential role in the activation of myosin light chain kinase, which phosphorylates myosin, thereby activating the actin-myosin interaction (for review see []).It has also be estimated that Ca 2+ influx under basal conditions is . It represented the largest isoactin gene characterized to that time, measuring over 23,000 bp from the transcription start site to the polyadenylation signal. Alpha-Smooth Muscle Actin Antibodies Antibodies that detect Alpha-Smooth Muscle Actin can be used in several scientific applications, including Immunohistochemistry, Western Blot, Immunocytochemistry, Flow Cytometry and ELISA. These cells produce the extracellular matrix (ECM) and are involved in many pathological changes in the vascular wall. 366 genes found i The search result lists the genes found by the search function. Results: In all, 320 and 313 genes were differentially expressed in bladder and corporal tissue, respectively. Smooth muscle alpha-actin (SMA) is the definitive marker for the activated cardiac fibroblast population known as the myofibroblast ( Frangogiannis et al., 2000 ), but this actin isoform is also abundant in vascular smooth muscle cells ( Skalli et al., 1989 ). Based on similarity to peptide cleavage of related actins, the mature protein of this gene is formed by removal of two N-terminal peptides. [provided by RefSeq, Dec 2010]. In humans, ACTA2 is encoded by the ACTA2 gene located on 10q22-q24. Myofibroblasts are. Myofibroblasts are identified by the neo-expression of -smooth muscle actin (-SMA) and the absence of desmin, smooth muscle myosin heavy chain (SM-MHC), h-caldesmon, and smoothelin distinguishing them from SMCs. Anti-alpha Smooth Muscle Actin antibody (GTX100034) | GeneTex alpha Smooth Muscle Actin antibody Cat No. [1] Myofibroblasts express alpha-smooth muscle actin and have a phenotype intermediate between fibroblasts and smooth muscle cells. Calculated MW 42 kDa. Actin isoforms differ slightly in their N-terminus and the sequences of each are perfectly conserved in higher vertebrates. In our study, SM1, SM2, and SMemb were all positive in the normal media, as is the case in human teenagers . Early studies of muscle contraction have . The primary antibody anti-alpha-Smooth Muscle Actin mouse monoclonal, 1A4/ASM-1 (Cat. The muscular tissue or muscles of animals are composed of specialized elongated cells called muscle fibers. Alternative splicing results in multiple transcript variants encoding distinct isoforms. These cells have fibers of actin and myosin which run through the cell and are supported by a framework of other proteins. (MON -RTU1004) Anti -Actin Smooth Muscle, clone 1A4 [Mouse] - MONOSAN - CiteAb. In addition, it has also been shown that smooth . Stains smooth muscle cells in vessel walls, gut wall and myometrium. Northern analysis of -smooth muscle actin and MLCK mRNA was performed using standard methods. Anti-Smooth Muscle Actin Antibody (B4) is recommended for detection of enteric smooth muscle -Actin and, to a lesser extent, -smooth muscle, -skeletal and -cardiac (sarcomeric) isoforms of Actin of mouse, rat, human and chicken origin by WB, IP, IF and IHC (P); non cross-reactive with cytoplasmic Actins Like striated muscle, smooth muscle can tense and relax. actin alpha 2, smooth muscle Normal Function Collapse Section The ACTA2 gene provides instructions for making a protein called smooth muscle alpha ()-2 actin, which is part of the actin protein family. Defects in the smooth muscle actin gene cause aortic aneurysm familial thoracic type 6. Actin proteins are important for cell movement and the tensing (contraction) of muscles. Myoepithelial cells in breast and salivary gland are also stained. . In most cases of Rhabdomyosarcoma, this antibody gives negative results whereas M. S. Actin is positive in the rhabdomyoblasts. Three -muscle actin isoforms are sequentially expressed during in vivo cardiac development. Smooth Muscle Definition Smooth muscle is a type of muscle tissue which is used by various systems to apply pressure to vessels and organs. To understand whether actin isoforms have specific functions during cardiac . 67 Skeletal muscle arises from the somites, which form adjacent to the neural tube beginning at about E8.0 in the mouse. Actin, Smooth Muscle antibody does not stain cardiac or skeletal muscle; however, it will stain myofibroblasts and myoepithelial cells. Among its related pathways are Development Slit-Robo signaling and Genotoxicity pathway . Alpha-smooth muscle actin (alpha-SMA) is the actin isoform that predominates within vascular smooth-muscle cells and plays an important role in fibrogenesis. With the progression of atherosclerosis, the expression of SM2 first decreases in the smooth-muscle cells in the intima followed by a decrease in the expression of SM1, whereas smooth-muscle -actin is well preserved . Muscular contraction is a fundamental phenomenon in all animals; without it life as we know it would be impossible. Smooth muscle actin ( Acta2) expression is largely restricted to smooth muscle cells, pericytes and specialized fibroblasts, known as myofibroblasts.
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