PDF | The capacity to induce tumour-cell specific apoptosis represents the most unique feature of the TNF receptor (TNFR) family member CD40. RhoGDI2 induces EMT in gastric cancer cells. apoptosis and suggest new directions for AML therapy. J. PEITC has been shown to promote apoptosis in breast cancer cells (Hahm and Singh 2011; Lee and Cho 2008; Tseng et al. Further studies have demonstrated that GLPs directly or indirectly mediate cancer cell death through diverse mechanisms ( 14 - 16 ). Induction of apoptosis by tumor necrosis factor (TNF) is modulated by changes in the expression and activity of several cell cycle regulatory proteins. 1997 Nov 1;57(21):4838-48. RhoGDI2 promotes tumor growth and malignant progression in gastric cancer [], and induction of EMT is associated with tumor progression and poor prognosis in gastric cancer [].We therefore examined whether RhoGDI2 may be involved in the control of EMT in gastric cancer cells using SNU-484 cells in which RhoGDI2, which is not normally expressed, was . Induction of apoptosis and cell cycle arrest by CP-358,774, an inhibitor of epidermal growth factor receptor tyrosine kinase . The combination treatment induces apoptosis and caspase-8 cleavage in MDA-MB-468 and CAMA-1 but not in MCF-7 cells and downregulation of caspase-8 blocked apoptosis. Evodiamine Induces Apoptosis in AGS and MKN45 Cells Since evodiamine has been reported to induce apoptosis in several types of cancer cell lines [ 4, 5, 6, 7, 8 ], it was investigated whether decreased cell viability was caused by apoptosis in the current study. TPA-induced apoptosis appears to be mediated through a p53-independent pathway, and the up-regulation of p21WAF1 and Bax may be the molecular mechanisms by which TPA induces apoptosis. As shown in Fig. Depending on the agent selected and the concentrations used, apoptotic events can be detected between 8-72 h post-treatment. 801. Successful elimination of cancer cells through nonsurgical methods is ultimately approached through induction of apoptosis. The apoptosis induced by DSF/Cu in pancreatic and breast cancer cells is autophagy dependent. Download Citation | Chinese dragon's blood ethyl acetate extract suppresses gastric cancer progression through induction of apoptosis and autophagy mediated by activation of MAPK and . Cell cycle analysis revealed that D/F4 and EA/P2 induced G2/M phase cell cycle arrest in MCF-7 cells. PFT induces apoptosis on cancer cells - flow cytometry study 7-Aminoactinomycin D (7-AAD staining) was used to detect cancer cell viability. A role of reactive oxidative species (ROS) is noted to contribute to Withaferin A induced apoptosis (Lee and Choi, 2016).This can be specific to the type of cells as Withaferin A treatment leads to ROS production by inhibition of mitochondrial respiration in human breast cancer cell line but not in normal human mammary epithelial cells (Hahm et al., 2011). Type VI secretion system-mediated cell death in Vibrio cholerae colonies occurs in two space- and time-differentiated phases that cause death along the colony rim and interior, respectively. Furthermore, IBC may inhibit the . The purpose of this study was to evaluate FasL expression in breast cancer and to elucidate the role of its signaling in different breast cancer cell lines. Other reports have indicated that chemotherapeutic agents trigger apoptosis by inducing release of cytochrome c from mitochondria. Apoptosis an active physiological process resulting in cellular self-destruction of unwanted cells is absent in cancer cells. siRNA-mediated inhibition of livin selectively blocks the growth of Livin-expressing cancer cells through induction of apoptosis. Recent. Therefore, IBC may induce apoptosis in MGC803 cells via the Akt and Erk signaling pathways. Induction of apoptosis has been associated with increased expression of the pro-apoptotic BAX and BIM proteins (Hahm and Singh 2011; Lee and Cho 2008; Tseng et al. The successful induction of apoptosis and cell cycle arrest at IC 50 values, were confirmed by flow cytometry as well as by the mRNA expression levels of the genes involved in these processes. The induction of apoptosis after p53 transfection for 12 and 24 h was studied by flow cytometry and the proportions of early apoptotic cells (annexin V-FITC positive and PI negative) were determined. GLPs have been demonstrated to induce suppression of cell growth and apoptosis in different types of cancer cells, with minimal negative effects on normal cells ( 13 ). Cell viability was assessed and the appropriate dose was determined . The ultrasound could penetrate the tissue and cell under some of conditions which directly changes the cell membranes permeability, thereby allowing the delivery of exogenous molecules into the cells in some degree. With evidence of above data it is suggested that nimbolide exhibit anticancer effect through its apoptosis-inducing property. In this report, we investigated whether TZT-1027 inhibited the growth of various human cancer cells, and the cell death caused by TZT-1027 was due to apoptosis. Many anti-cancer agents halt cell cycles [ 9 - 12 ], which induces apoptosis in cancer cells. In addition, we elucidated the apoptosis machinery induced by treatment . Download Apoptosis And Cancer Therapy books, Since most cancer therapies primarily act by inducing apoptosis in tumor cells, insights into the molecular mechanisms regulating apoptosis are crucial to developing novel, more effective treatment strategies. In conclusion, the results of the present study demonstrated that IBC was capable of inhibiting the proliferation of gastric cancer cells and the induction of apoptosis in a concentration- and time-dependent manner. Here, a highly distinguished team of authors from top institutes around the world leads readers from the principles of programmed cell death . Autophagy activation is one of the . We found that knockdown of Blimp-1 expression by lentiviral vector-delivered short hairpin RNA causes . There are many ways a malignant cell can acquire reduction in apoptosis or apoptosis resistance. Abstract. Nonsteroidal anti-inflammatory drugs (NSAIDs) have shown cancer preventive activity in patients who took them frequently. Apoptosis is a highly controlled physiological mechanism and proceeds through two major pathways for apoptosis-inducing. J D Moyer 1, E G Barbacci, K K Iwata, L Arnold, B Boman, A Cunningham, C DiOrio, J Doty, M J Morin, M P . Hence, search for substances of natural origin having preferential cancer cell killing ability is of paramount importance Manipulating apoptosis is one avenue through which scientists can address a number of vexing medical problems. The induction of apoptosis, demonstrated by annexin V-FITC labeling, PARP cleavage and mitochondrial membrane depolarization and morphological alterations, were clearly detected in MCF7 ADR and MDA-MB-231 and to a minor extent in MCF7. Phoenix dactylifera L. have been mentioned in folk remedies of traditional Egyptian medicine and alternative medicine, for numerous health benefits including cancer treatment. In pancreatic and ovarian cancer cells, the induction of necroptosis plays a EP 67 significant role in their killing [14, 15]. Majid Mahdavi. Cancer Res.93, 874- 882, August 2002 874 Induction of Apoptosis in Hepatocellular Carcinoma Cell Lines by Emodin Xubin Jing,1, 2 Noboru Ueki,1 Jidong Cheng,1, 2 Hiroyasu Imanishi1 and Toshikazu Hada1, 3 1Division of Hepatobiliary and Pancreatic Disease, Department of Internal Medicine, Hyogo College of Medicine, 1-1 Mukogawacho, Nishinomiya, Hyogo 663-8501 and 2Shantou University . Scratch assays indicated the inhibition of cell migration in the cancer cell lines treated by Streptomyces sp. 2004). These drugs can induce tumor cells to undergo apoptosis in vitro. A major challenge in designing cancer therapies is the induction of cancer cell apoptosis, although activation of intrinsic apoptotic pathways by targeting gold nanoparticles to mitochondria is promising. Bilberry extract induced apoptotic cell bodies and nucleosomal DNA fragmentation in HL60 cells. Although the develop-ment of better chemotherapy regimens has improved remission induction and overall survival, relapse remains a common prob-lem, especially among older patients and or patients with poor Thus, all designers of cancer drugs to try either to rectify defective apoptotic mechanism or activated inactivated one. Several reports have suggested that anticancer drugs kill susceptible cells by inducing expression of death receptor ligands, especially Fas ligand (FasL). After extensive cell membrane blebbing occurs, it will be followed by the formation of apoptotic bodies through the separation of cell fragments.22 Annexin V-FITC is an important marker for confirmation of apoptosis in cells due to the presence of an intact cell membrane in the early phase of apoptosis.22 This condition allows Annexin V-labeled FITC to be bound to the outer membrane of the . Extract T7 was not mutagenic. 4 , early apoptosis was clearly detected in HeLa and PC-3 cells for all the p53 transfection groups after 12 h incubation. Ultrasound could inhibit the proliferation or induce the apoptosis of the cancer cell in vitro or in vivo. Induction of Apoptosis in Human Breast Cancer Cells via Caspase Pathway by Vernodalin Isolated from Centratherum anthelminticum (L.) Seeds Chung Yeng Looi, 1 , * Aditya Arya, 1 , 2 Foo Kit Cheah, 1 Bushra Muharram, 1 Kok Hoong Leong, 2 Khalit Mohamad, 2 Won Fen Wong, 3 Nitika Rai, 4 and Mohd Rais Mustafa 1 Xiaolin Zi, Editor Acute myelogenous leukemia (AML) is a serious and often lethal form of hematologic cancer. Necroptosis could also be used as an adjuvant for cancer 68 therapy, because it triggers a . Ethnopharmacological relevance: Phoenix dactylifera L. (Ajwa date) has high nutritive value and are consumed in Arabian Peninsula as an essential diet. On the other hand, MDA-MB-231 cells treated with D/F4 and D/F5 accumulated in the sub-G Leptin treatment causes cancer cell growth via stimulating various signaling pathways. From these results, we conclude that TPA is not only a tumor promoter, but also induces apoptosis in breast cancer cells. Apoptosis is characterised by distinct morphologic changes, including cell shrinkage, membrane blebbing, chromatin condensation, DNA fragmentation, and the formation of apoptotic bodies [ 12 ]. Human leukemic cells were highly sensitive to ET-18-OCH 3, whereas normal cells remained unaffected. Non-small cell lung cancer (NSCLC) is the leading cause of death in lung cancer due to its aggressiveness and rapid migration. Cancer Res. MeSH terms Apoptosis / drug effects* Recent research indicated low frequency and low intensity ultrasound . NS398, a cyclooxygenase-2 (COX-2)-selective inhibitor, has been reported to cause apoptosis in cancer cell lines. The induction of cell death through apoptosis by the active fractions on the breast cancer cells was confirmed by Annexin V-FITC and PI staining. Guidelines for the use and interpretation of assays for monitoring autophagy | Find, read and cite all the research you . Methods T47D and MCF7 cells were used and cultured in Dulbecco's modified Eagle's medium. Induction of apoptosis in cancerous cells is one of the strategies of modern cancer therapeutic regimes. Some anticancer drugs kill cancer cells by inducing apoptosis via death receptor . This study aimed to evaluate the effect of NaBu on cell cycle control, apoptosis, and expression of some lncRNAs in two human prostate cancer cells (PC-3 and LNCAP). Extract T7 induced death by apoptosis and cell cycle arrest in the G 2 phase in C-33 A. Induction of marked apoptosis in mammalian cancer cell lines by antisense DNA treatment to abolish expression of DENN (Differentially Expressed in Normal and Neoplastic Cells) Molecular Carcinogenesis Apoptosis is preceded by association of RIP1 with caspase-8. Poly (ADP-ribose) polymerase-1 (PARP1) is a major member of the PARP superfamily that is involved in DNA damage signalling and other important cellular processes. Here we report the development of a small molecule targeting PARP1 based on the PROTAC strategy. 2015, Acta Biochimica Polonica . However, limited options are available with severe side effects. Chemical induction of apoptosis Apoptosis inducers act on several apoptosis-related proteins to promote apoptotic cell death. Purpose The Fas-antigen is a cell surface receptor that transduces apoptotic signals into cells. Inhibition of cell proliferation and induction of apoptosis in K562 human leukemia cells by the derivative (3-NpC) from dihydro-pyranochromenes family. Exposure of H661 cells to 30 microM EGCG, EGC or theaflavins for 24 h led to the induction of apoptosis as determined by an annexin V apoptosis assay, showing apoptosis indices of 23, 26 and 8%, respectively; with 100 microM of these compounds, the apoptosis indices were 82, 76 and 78%, respectively. ( a) Colony formation assays of H1299 cells (which do not. FasL translocation to the membrane was achieved . Further investigation revealed disruption of mitochondrial membrane potential orchestrated with induction of reactive oxygen species leading to the activation of caspases, key players of apoptotic cell death. Conclusions Sodium butyrate (NaBu) is a short-chain fatty acid acting as a histone deacetylase inhibitor, and has been shown to be a potential regulator of cancer cell death. Downregulation, but not kinase inhibition, of receptor-interacting protein 1 (RIP1) suppressed apoptosis in CAMA-1. Introduction Breast cancer is the most common malignancy in women. HMC induced caspase-dependent apoptosis in MCF-7 cells, which was associated with its ability to modulate a series of cell survival-related signaling effectors, including Akt, mTOR, Bax, Mcl-1, and Bcl-2. First, PPZ selectively induced apoptosis in cancer cells, and this PPZ-induced apoptosis may be caused by suppressing ERK phosphorylation. Jpn. 3.1.1 Reactive oxidative species. Some reviews have revealed that apoptosis-inducing was a promising therapy in cancers with the least side effects and more effectiveness. In this study, we tested whether the formulation exerts its anti-tumor effects through induction of apoptosis on prostate cancer cell line DU-145. Because cells can react to abnormal growth stimuli, such as those exerted by the E7 oncoprotein and pRb mutations, by inducing apoptosis ( 19 - 22 ), it is possible that this cellular response is blocked in HPV-positive cancer cells by the antiapoptotic potential of the multifunctional E6 protein. Additionally, Extract T7 exhibited a cytotoxic effect against cervical cancer cells, where C-33 A was the most sensitive (IC 50 = 22.58 1.10 g/mL and 14.26 1.11 g/mL at 24 h and 48 h respectively). Since anticancer agents are known to induce apoptosis in cancer cells and apoptosis biomarkers are being increasingly used in clinical trials [ 37 ], a total of 13 extracts showing significant cytotoxicity were tested for their proapoptotic potential in HeLa cells by using APOPercentage assay. Nimbolide induces the cleavage of pro-caspase-8, pro-caspase-3 and PARP. Vernodalin inhibited cell growth of human breast cancer cells MCF-7 and MDA-MB-231 by induction of cell cycle arrest and apoptosis. Apoptosis, Flavonoids, Cells Abstract Among ethanol extracts of 10 edible berries, bilberry extract was found to be the most effective at inhibiting the growth of HL60 human leukemia cells and HCT116 human colon carcinoma cells in vitro. B lymphocyte-induced maturation protein-1 (Blimp-1) is a transcriptional repressor that plays an important role during plasmacytic differentiation and is expressed in normal and transformed plasma cells. Proposed model for autophagy induction by leptin and its role in suppression of apoptosis in cancer cells. Increased of reactive oxygen species (ROS) production, coupled with downregulation of anti-apoptotic molecules (Bcl-2, Bcl-xL) led to reduction of mitochondrial membrane potential (MMP) and release of cytochrome c . The potent antitumor effect of Smp24, an antimicrobial peptide derived from Egyptian scorpion Scorpio maurus palmatus via Employing the intrinsic pathway, cancer cells, cells that are infected with bacteria or virus particles, and mutant cells can be assigned to apoptosis.The extrinsic pathway is commonly associated with cellular death receptors.. Apoptosis in medicine. This H + /K + -ATPase inhibitor simultaneously stimulated phosphorylation of p38 as well as deactivation of ERK in a dose- and time-dependent manner only in cancer cells. Induction of apoptosis in human breast cancer cells by nimbolide through extrinsic and intrinsic pathway P.Elumalai D.N.Gunadharini K.Senthilkumar S.Banudevi R.Arunkumar C.S.Benson G.Sharmila J.Arunakaran https://doi.org/10.1016/j.toxlet.2012.10.008 Get rights and content 1. The effect of the nutrient mixture (NM) on cell growth inhibition in DU-145 cells was examined by 3- (4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. 2004) and to decrease ER expression (Kang and Wang 2010). We here investigated the importance of continuous Blimp-1 expression. Apoptosis in colorectal tumour cells: Induction by the short chain fatty acids butyrate, propionate . Cell growth stimulation could manifest in conditions where cell survival is enhanced and cell death is inhibited. Here we have found that Et-18-OCH 3 induced apoptosis not only in human tumor cell lines but also in primary tumor cell cultures from cancer patients. However, not all reagents will affect a particular cell line in the same way. From these results, we conclude that TPA is not only a tumor promoter, but also induces apoptosis in breast cancer cells. The above data suggest that nimbolide induces apoptosis by both the intrinsic and extrinsic pathways.
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