cd44 molecular weightagrometeorology instruments

Molecular weight (Da) 79210.82 Theoretical pI 5.10 CD44 Protein Structure Hyaluronan binding domain of human CD44 Deposited 2003-12-19 Released: 2004-03-04 Deposition Author (s) CD44 has been referred to as HCAM (homing cell adhesion molecule), Pgp-1 (phagocytic glycoprotein-1), Hermes antigen, lymphocyte homing receptor, ECM-III, and HUTCH-1. As can be seen from the image ( Figure 5 ), the band B2 is the most extensive. When cultured in the presence of ULMW-HA (6.4kDa), but not of LMW-HA (31kDa) or HMW-HA (980kDa), thymidine uptakes of cell lines expressing CD44 at very high levels (KOPB-26 . HMWH attenuated mechanical hyperalgesia induced by the inflammatory mediator prostaglandin E2 (PGE2) in male and female rats. Glioblastoma multiforme (GBM), a highly invasive brain tumor, is associated with aberrant HA secretion, tissue stiffening, and overexpression of the HA receptor CD44. CD44 is highly polymorphic, possesses a number of alternative splice variants and undergoes extensive post-translational modifications (1,2). Low molecular weight hyaluronan (LMWH) acts at both peptidergic and nonpeptidergic nociceptors to induce mechanical hyperalgesia that is prevented by intrathecal oligodeoxynucleotide antisense to CD44 mRNA, which also prevents hyperalgesia induced by a CD44 receptor agonist, A6. CD44 is a family of non-kinase, single span transmembrane glycoproteins expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow [1, 2].CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (CSC) [].In humans, CD44 is encoded by 19 exons with 10 of these . CD44 is highly polymorphic, possesses a number of alternative splice variants and undergoes extensive post-translational modifications (1,2). Ongoing LMWH and A6 hyperalgesia are reversed by HMWH. Next, we investigated the impact of the molecular weight of HA on the derivative binding to CD44. Hyaluronan is a high molecular weight (10 5 to 10 7 Da) GAG composed of alternating N-acetyl-glucosamine (GlcNAc) and glucuronic acid (GlcA) . CD44s is a single-chain molecule composed of a distal extracellular domain (containing, the ligand-binding sites), a membrane-proximal region, a transmembrane-spanning domain, and a cytoplasmic tail. NMR spectra were shown in Fig. . Intrathecal administration of an oligodeoxynucleotide antisense (AS-ODN) to mRNA for cluster of differentiation 44 (CD44 . Anti-CD44 antibody [EPR1013Y] staining . CD44 Signaling Mediates High Molecular Weight Hyaluronan-Induced Antihyperalgesia. In humans, the CD44 antigen is encoded by the CD44 gene on chromosome 11. The human CD44 gene is located on the short arm of chromosome 11 and has a full length of about 50 kb. CD44 is the major surface hyaluronan (HA) receptor implicated in intercellular and cell-matrix adhesion, cell migration and signaling. Low molecular weight hyaluronan (LMWH) acts at both peptidergic and nonpeptidergic nociceptors to induce mechanical hyperalgesia that is prevented by intrathecal oligodeoxynucleotide antisense to CD44 mRNA, which also prevents hyperalgesia induced by a CD44 receptor agonist, A6. After treatment with either molecular weight of HA, the amount of free (non-HA bound) CD44S is still ~90%. 1 author 2. The High and Low Molecular Weight Forms of Hyaluronan Have Distinct Effects on CD44 Clustering* CD44 is a major cell surface receptor for the glycosaminoglycan hyaluronan (HA). Product Specific Protocols; WB protocol for CD24 antibody 10600-1-AP: Download protocol: Standard Protocols; . Target Information CD44 cell surface antigen is a 100 kDa type 1 transmembrane glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. The CD44 antigen is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. In order to estimate the availability of the binding partners of CD44 and of HA, the stoichiometry of the binding interaction was calculated for each HA size as follows. In this study, we investigated LMW-HA-mediated CD44 interaction with Toll-like receptors (TLRs), the actin filament-associated protein (AFAP-110), and a myeloid differentiation factor (MyD88) in breast tumor cells (MDA-MB-231 cells). Statistics analysis Data were presented as the mean SD or median with interquartile range (IQR). The gene for CD44 has been identified in human, mouse and rat. Since it is highly glycosylated, the apparent molecular weight of CD24 could be variable, ranging from 30 kDa to 70 kDa. The delineation of membranes was based on the well-known molecular weight of investigated proteins. 15675-1-AP. Both effects are dependent on the binding of LMW-HA to its receptor, CD44, and ultimately promote BM development. CD44 is a major cell surface receptor for the large polydisperse glycosaminoglycan hyaluronan (HA). CD44, a non-kinase cell surface transmembrane glycoprotein, has been widely implicated as a cancer stem cell (CSC) marker in several cancers. CD44 Polyclonal antibody. Thus the extensive sulfation associated with the high . Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. In addition, preparations of HA defined operationally as "low molecular weight" (20-500 kDa) have been reported to promote cell cycle progression via a CD44/Rac/ERK pathway in smooth muscle cells, whereas higher molecular weight fractions (>500 kDa) were shown to be inhibitory . Effect of molecular weight on binding of HA derivatives. Article CAS Google Scholar This protein has been implicated in cancer aggressiveness, metastasis and growth. CD44 is a hyaluronic acid receptor that regulates cell repair, adhesion and inflammation. Anti-CD44v6 Antibody, exon v6 is an antibody against CD44v6 for use in ELISA, FC, IP, WB, IC, IH(P). Binding of the long and flexible HA chains is thought to be stabilized by the multivalent nature of the sugar molecule. As the figure 1 shows: Figure 1. Given the expected molecular weight of CD44-Fc (170 kDa) in a dimer form and 1 RU = 1 pg/mm 2 protein relationship [30] we estimated surface density of ~ 3 receptors per 1000 nm 2. WB 1/5000. Anti-CD44 antibody [EPR18668] (ab189524) Research with confidence - consistent and reproducible results with every batch Long-term and scalable supply - powered by recombinant technology for fast production Success from the first experiment - confirmed specificity through extensive validation Ethical standards compliant - production is animal-free Thus, a higher expression of CD44 can be correlated in cells treated with solution B, i.e. CD44-deficient macrophages showed reduced motility in a Transwell migration assay, reduced ability to secrete the proinflammatory cytokine TNF-, . Surgically resected specimen was fixed in 10% buffered formalin and embedded in paraffin. The bands represent the expression of CD44 before and after exposure with solutions A and B. Conversion of 1-742 amino acid of CD44 mRNA gives the mass of 84kDa. MA5-15462 detects CD44 which has a predicted molecular weight of approximately 82kDa. Target Information CD44 cell surface antigen is a 100 kDa type 1 transmembrane glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Ongoing LMWH and A6 hyperalgesia are reversed by HMWH. (DMPE) to the carboxylic residues of HA of different molecular weight (6.4 . CD44 (CD44 Molecule (Indian Blood Group)) is a Protein Coding gene. CD44s has a molecular weight of around 84kDa. Diseases associated with CD44 include Malignant Pleural Mesothelioma and Lymphoma.Among its related pathways are Apoptotic Pathways in Synovial Fibroblasts and Development Beta-adrenergic receptors regulation of ERK.Gene Ontology (GO) annotations related to this gene include transmembrane signaling receptor activity and . There is no significant difference in the amount of unoccupied CD44S, regardless of HA molecular weight. CD44v6 (Approximant 200-250 kDa) Immunogen. Features and Benefits Cells that lacked membranous staining were considered negative. Thus, CD44 is used as a cell-surface marker for several cancers . with hyaluronic acid of molecular weight between 50- 2500 kDa. We evaluated the mechanism by which high-molecular-weight hyaluronan (HMWH) attenuates nociceptor sensitization, in the setting of inflammation. It remains unclear whether the fragment size of HA can regulate the . Notes; IHC-P 1/50 - 1/100. Molecular Weight: 80-95 kDa. One HA receptor, the cluster of differentiation CD44 protein, is often overexpressed in tumor cells, As such, HA has attracted considerable interest in the development of drug delivery formulations, given its intrinsic targetability towards CD44 overexpressing cells. CD44(960) Description. It is a transmembrane, highly glycosylated protein with several isoforms resulting from alternative gene splicing. Anti-CD44 antibody is specific for CD44 in humans. Molecular weight specific impact of soluble and immobilized hyaluronan on CD44 expressing melanoma cells in 3D collagen matrices. with weight molecular ranging from 1,000- 1,800 kDa and hyaluronic acid with weight molecular comprised between 13, no. Epitope: exon v6. . The molecular sequence (with the exception of the membrane-proximal region) displays high interspecies homology. The human CD44 gene encodes multiple isoforms of a transmembrane protein that differ in their extracellular domains as a result of alternative splicing of its variable exons. Synthetic peptide of human CD44v6. PKH26-labeled CD44 or scrabbled siRNA-treated MSC EV (90 l) incubated with HMW HA (1.0 MDa) were intravenously instilled 4 h following pneumonia. Its variant usually has a higher molecular weight. However, chondroitin ABC and chondroitin AC lyase digestion of CD44 immunoprecipitated from HTB58 cells reduced the apparent molecular mass of the 90- and 180-kDa forms of CD44 (Fig.7, B and C) and resulted in the release of sulfate from the high molecular weight species of CD44 (Fig. Acta Biomater 50 , 259-270 (2017). The high-molecular-weight glycosaminoglycan, hyaluronic acid (HA), makes up a significant portion of the brain extracellular matrix. CD44: molecular interactions, signaling and functions in the nervous system. Ferrari LF1, Khomula EV1, Araldi D1, Levine JD2 Author information Affiliations 3 authors 1. As a result, CD44 molecules range in molecular weight from 80 to over 200 kDa . The smallest isoform, known as hematopoietic or standard CD44 (CD44s), is expressed on the surface of most mammalian cell types and contains none of the alternatively spliced exons. CD44 is a major cell surface receptor for the glycosaminoglycan hyaluronan (HA). "Structure of the regulatory hyaluronan binding domain in the inflammatory leukocyte homing receptor CD44," Molecular Cell, vol. Increased surface levels of CD44 are characteristic of T cell activation, and expression of the protein is upregulated during the inflammatory response. CD44 is ubiquitously expressed throughout the body and has a molecular weight of 85-200 kDa ( Basakran, 2015 ). Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa). High-molecular-weight (HMW)-HA which exists as extracellular matrix in tissues is incorporated into cells in a CD44-dependent manner, and then is secreted from cells as low-molecular-weight (LMW) to ultra-low-molecular-weight (ULMW)-HA in normal and pathological conditions. The location of CD44 gene 2.1 The gene structure of CD44 Low molecular weight hyaluronan (LMWH) acts at both peptidergic and nonpeptidergic nociceptors to induce mechanical hyperalgesia that is prevented by intrathecal oligodeoxynucleotide antisense to CD44 mRNA, which also prevents hyperalgesia induced by a CD44 receptor agonist, A6. ELISA: 1:1000-1:2000 Flow Cytometry: 1:500-1:1000 Specificity. This full length includes the . Native high molecular weight hyaluronan (nHA) and oligosaccharides of hyaluronan (oHA) provoke distinct biological effects upon binding to CD44. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa). Comment: CD44 is a marker for cancer stem cell and a biomarker for embryonic stem cells. Both high and low molecular weight hyaluronan (HMW-HA vs. LMW-HA) exist in various tissues and cells. CD44 categorizes a huge family of transmembrane glycoproteins, belonging to the class of cell adhesion molecules [24]. 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