elife (2016) ncbi Bioz Stars score: 86/100, based on 1 PubMed citations. [supplied by OMIM] Limitations While ZEB proteins mainly function as transcriptional suppressors, they are able to activate transcription, dependent on DNA-context and cell type (1). Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Immunoprecipitation of ZEB1 protein from Jurkat cell extracts. 40 ug 293T whole cell lysate/extract B. Monoclonal Antibody for studying ZEB1/TCF8. There are two members in mammals, ZEB1 (-EF1, TCF8, AREB6) and ZEB2 (SIP1). ZEB1 is a transcriptional repressor of interleukin-2 (IL-2). These antibodies target ZEB1 in Human, Mouse and Rat samples. One of the targets suppressed by ZEB proteins is E-cadherin. ZEB1 is a zinc finger transcription factor that represses T-lymphocyte-specific IL2 gene expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site. This target gene encodes the protein 'zinc finger E-box binding homeobox 1' in humans and may also be known as zeb, AREB6, TCF8, BZP, DELTAEF1, and delta-crystallin enhancer binding factor 1. Cell Signaling Technology Inc rabbit monoclonal anti zeb1 Rabbit Monoclonal Anti Zeb1, supplied by Cell Signaling Technology Inc, used in various techniques. The ZEB1 (D80D3) Rabbit mAb Antibody from Cell Signaling Technology is a Rabbit Monoclonal antibody to Zeb1. Our ZEB1 monoclonal, polyclonal and recombinant monoclonal antibodies are developed in Mouse, Rabbit and Goat. All lanes : Anti-ZEB1 antibody [BLR102H] (ab276129) at 1/1000 dilution Lane 1 : C2C12 whole cell lysate Lane 2 : 70Z/3 whole cell lysate Lane 3 : BW5147.3 whole cell lysate Lane 4 : CTLL-2 whole cell lysate Lane 5 : EL4 whole cell lysate Lane 6 : RenCa whole cell lysate Lane 7 : NIH/3T3 whole cell lysate Lane 8 : TCMK-1 whole cell lysate Lane 9 : A20 whole cell lysate . ZERO BIAS - scores, article reviews, protocol conditions and more Anti-ZEB1 antibody . Anti-Zeb1 Antibody Products from Cell Signaling Technology Anti-ZEB1 antibodies are offered by a number of suppliers. Background ZEB family proteins are zinc-finger and homeobox domain containing transcription factors. #9782. Bioz Stars score: 96/100, based on 2 PubMed citations. ABclonal provides trial size antibody samples for target detection. Bioz Stars score: 95/100, based on 1 PubMed citations. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. EMT induced by loss of LKB1 promotes migration and invasion of liver cancer cells through ZEB1-induced YAP signaling Qiu B et al Oncol Lett 2018;16(5):6465-6471 Monoclonal antibody is produced by immunizing animals with recombinant protein corresponding to the central region of human ZEB1 protein. Tcf8 Zeb1 Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. This Cell Signaling Technology antibody is conjugated to Alexa Fluor 647 fluorescent dye and tested in-house for direct flow cytometric analysis in human cells. ZERO BIAS - scores, article reviews, protocol conditions and more In order to research induction of epithelial-to-mesenchymal transition by Polo-like kinase 1 and promotion of epithelial cell motility by activating CRAF/ERK signaling, ProSci ZEB1 antibody (ProSci Inc, 5825) was used in western blot on human samples at 1:250 (fig 5). Validated for WB, IP, IHC, IHC, IF, F. Available in 2 sizes. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of ZEB1 protein by 2 ug of . ZEB1 antibodies are useful tools for studying certain degenerative ocular diseases. ZERO BIAS - scores, article reviews, protocol conditions and more. Anti Zeb1 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Senescent neutrophils secret exosomal piR-17560 in a STAT3-dependent manner. We are dedicated to providing innovative research tools that are used to help define mechanisms underlying cell function and disease. . Limitations. Primary antibody was incubated over night at 4 C (active -catenin 1:1000 in 5% BSA/TBST (non-phospho/active -catenin, Ser33/37/Thr41, rabbit mAb; Cell Signaling, Danvers, MA, USA)). Beta Catenin in Cell Adhesion and T-cell Signaling Beta Catenin is a cytosolic, 88 kDa intracellular protein that tightly . Monoclonal Rabbit Anti Zeb1, supplied by Cell Signaling Technology Inc, used in various techniques. ZEB1 encodes a zinc finger transcription factor that represses T-lymphocyte-specific IL2 gene (MIM 147680) expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site (Williams et al., 1991 [PubMed 1840704]). Bioz Stars score: 96/100, based on 1 PubMed citations. Since its inception, CST has become the world . Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. . Background ZEB family proteins are zinc-finger and homeobox domain containing transcription factors. Cell Signaling Technology Inc anti zeb1 3396s antibodies Anti Zeb1 3396s Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Beta Catenin in Cell Adhesion and T-cell Signaling Beta Catenin is a cytosolic, 88 kDa intracellular protein that tightly associates with cell . Antibodies that detect ZEB1 can be used in several scientific applications, including Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation and ChIP. 100% Guaranteed. Western blot analysis was performed using ZEB1 (E2G6Y) XP Rabbit mAb. human ZEB1 antibody; mouse Zeb1 antibody; rat Zeb1 antibody; browse more . Replacement to Abcam, Santa Cruz, Sigma and CST antibody. This antibody recognizes Human antigen. Monoclonal antibody is produced by immunizing animals with recombinant protein corresponding to the central region of human ZEB1 protein. Share by email . Product Usage Information Storage Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger. Validations Orthogonal Applications Cited in 20 publications. Bioz Stars score: 96/100, based on 1 PubMed citations. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP Isotype Control #3900, and lane 3 is ZEB1 (E2G6Y) XP Rabbit mAb. ZEB1 and ZEB2 contain two separate zinc-finger domains and a homeodomain (1). ( A ) miR-205-NPs treatment suppresses the migratory ability of C4-2 and PC-3 cells in presence/absence of the drug through Boyden chamber study. Home > Search Results > Cell Signaling Technology Inc > tcf8 zeb1 antibodies. ZERO BIAS - scores, article reviews, protocol conditions and more. Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. ZERO BIAS - scores, article reviews, protocol conditions and more The enriched DNA was quantified by real . ZEB1 (zinc-finger E-box-binding homeobox 1), also known as TCF8 (transcription factor 8), is a delta-EF1/ZFH-1 C2H2-type zinc finger that bears seven C2H2-type zinc fingers and one homeobox DNA-binding domain. In mice, Zeb1 induced increased cadherin and Integrin expression in the gastrocnemius muscle following an injection with an adenovirus vector expressing Nr4a1(Nr4a1 . Epithelial-Mesenchymal Transition (EMT) Antibody Sampler Kit. Antibody information for antibodies HPA027524, CAB058686 and CAB079943 used in analysis of ENSG00000148516 / ZEB1 (AREB6, BZP, FECD6, NIL-2-A, PPCD3, TCF8, ZEB, Zfhep, Zfhx1a) . Available in 2 sizes. Moreover, Nr4a1 increased myoblast adhesion via directly binding to an upstream site of zinc finger E-box binding homeobox 1 (Zeb1), which is required for myogenesis in myoblasts. B Cell Receptor Signaling Pathway; T Cell Receptor Signaling Pathway; Jak-Stat-IL-6 . This product is for research use only and is not approved for use in humans or in clinical diagnosis. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated ZEB1 (E2G6Y) XP Rabbit mAb #70512. Established in Beverly, MA in 1999, Cell Signaling Technology (CST) is a privately-owned company with over 400 employees worldwide. Log in / Register . 8776162355. headquarters: USA. Please check that this product meets your needs before purchasing. The ZEB1 (D80D3) Rabbit mAb Antibody has been validated for the following applications: Precipitation, Immunoprecipitation, and Western Blot. ZEB1 Modification Unmodified View Product On Supplier's Website Request a Quote from Cell Signaling Technology Supplier provided information Immunogen Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to a region around residue Asp868 of human TCF8/ZEB1. Cited in 354 publications. Total cell lysates were prepared in NP-40 lysis buffer as described. Western Blot analysis of ZEB1 in HeLa cell lysate with ZEB1 Polyclonal Antibody (Product # PA5-20979) at 1 g/mL. Home > Search Results > Cell Signaling Technology Inc > anti zeb1 antibody. Cell Signaling Technology Inc polyclonal zeb1 antibody Polyclonal Zeb1 Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Anti-ZEB1 antibody [EPR17375] (ab203829) Research with confidence - consistent and reproducible results with every batch Long-term and scalable supply - powered by recombinant technology for fast production Success from the first experiment - confirmed specificity through extensive validation Ethical standards compliant - production is animal-free Figure 6 miR-205-NPs inhibits EMT signaling and sensitizes Dtxl treatment in PrCa cells. . Subsequently, the samples were washed thoroughly using TBST and incubated for 1 h at room temperature with a secondary antibody goat anti-rabbit IRDye800CW (1 . Highly specific and rigorously validated in-house, ZEB1 (E2G6Y) XP Rabbit Monoclonal Antibody (CST #70512) is ready to ship. Immunoprecipitation: ZEB1 Antibody [NBP2-20942] - 293T whole cell lysate/extract A. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as the secondary antibody. Validated for Western Blotting, Immunoprecipitation. zeb1 antibody (Cell Signaling Technology Inc) 94. Bioz Stars score: 96/100, based on 17 PubMed citations. All lanes : Anti-ZEB1 antibody (ab245283) at 0.1 g/ml Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell . Highly specific and rigorously validated in-house, ZEB1 (D80D3) Rabbit Monoclonal Antibody (CST #3396) is ready to ship. 33 Soluble proteins were subjected to immunoprecipitation with anti-ZEB1 antibody (Cell Signaling Technology, Danvers, MA, USA). There are two members in mammals, ZEB1 (-EF1, TCF8, AREB6) and ZEB2 (SIP1). Chromatin immunoprecipitations were performed with cross-linked chromatin from HCT 116 cells and either -Catenin (D10A8) XP Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. 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